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What are the methods for isolation and purification
Affinity chromatography is one of the most commonly used methods of separation and purification in recent years. Many substances have strange focal points and reversible properties of compounds.
Then use the appropriate method to make biological macromolecular separation and elution from ligand, so as to achieve the purpose of separation and purification (for example, enzyme and enzyme and substrate and coenzyme (products or competitive inhibitors, etc.), antigen and antibody, in the cold receptors, vitamins and proteins, lectins and polysaccharide (or glycoprotein or cell surface receptors), nucleic acids and complementary strand (or set of protein or nucleic acid polymerase or binding protein) and cell surface proteins (or agglutinin).
Affinity chromatography is a chemical method and technique that is separated from the reversibly specific composite (called ligand) of the material to the continuous solid carrier, and the ligand column containing the solid phase carrier. When the biomolecules are purified by the chromatographic column, the biomolecules are bound to the specific carrier ligand, other materials are eliminated.
The new trend of development of chromatography in the field of biological pharmacy